EVALUATION OF THE STABILITY AND THE ENZYMATIC ACTIVITY OF PAPAIN SOLUTIONS USED IN THE DEBRIDEMENT AND WOUND HEALING
Abstract
Papain solutions (2 % w/v, 6 % w/v and 10 % w/v) used in debridement and wound healing, were prepared without and with pharmaceutical adjuvants (propylene glycol, EDTA and methyl paraben) and were stored in environments
with different temperatures (5-10 ºC and 30-35 oC). Some analyses were performed during the storage: organoleptic analysis, pH, microbiological contamination and enzymatic activity (milk-clotting method). The results showed that pH and organoleptic aspects are not reliable guides to monitor changes in enzymatic activity in the solutions. There were no problems concerning microbiological contamination of the solutions during the evaluation period. Storage environments with lower temperatures can maintain high enzymatic activities of the solutions. There was not a proportional
increase of the maximum enzymatic activity of the solutions without adjuvants and the concentration of papain in the formulations. Initially, the papain solutions without adjuvants exhibited enzymatic activity three to five times
higher than the other samples, but there was rapid decrease. Therefore, papain solutions should be used in the form of extemporaneous preparations in dressings. On the other hand, solutions with adjuvants have their enzymatic activities preserved for a longer time.
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PDF (Português (Brasil))DOI: http://dx.doi.org/10.14450/2318-9312.v28.e3.a2016.pp179-184
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Copyright (c) 2016 Júlio Cézar Borella, Renata Filliettaz Simões, Robison Leandro Aparecido Puga, Marise C. Bastos Stevanato
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Infarma - Ciências Farmacêuticas
ISSN - 2318-9312 (Versão eletrônica)
ISSN - 0104-0219 (Versão impressa)
Conselho Federal de Farmácia - CFF
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CEP: 71635-615 - Fone: (61)3878-8751
e-mail:infarma@cff.org.br
Diretoria
Walter da Silva Jorge João, Presidente do Conselho Federal de Farmácia
Lenira da Silva Costa, Vice-Presidente do Conselho Federal de Farmácia
João Samuel de Morais Meira, Tesoureiro do Conselho Federal de Farmácia
Luiz Gustavo de Freitas Pires, Secretário-Geral do Conselho Federal de Farmácia